Exhibit 99.1
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Artificial Intelligence Neural Network for Target Prediction Revolutionizing Next-Gen Allogenic CAR Therapy for Solid Tumors |
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Disclaimer This presentation is provided by Kiromic BioPharma, Inc. (the “Company”) for the express purpose of giving prospective investors, bankers, employees, consultants, and corporate affiliations information regarding the Company to assist the recipi-ent in evaluating a potential formal associa-tion with the Company. While the informa-tion contained herein or in any other mate-rials that may be provided by the Company is believed to be true, accurate and reason-able, the Company makes no such represen-tation or warranty, express or implied, as to the veracity, accuracy, reasonableness or completeness of such information. The Company expressly disclaims any and all liability which may be based on such infor-mation, any errors therein or omissions therefrom. This presentation does not imply an offering of Securities. This presentation may contain forward-looking statements within the meaning of applicable securities regulations. All statements other than state-ments of historical facts are forward-looking statements. In some cases, forward-looking statements may be identified by the use of words such as "anticipate," “believe,” "plan," “estimate,” "expect," "intend," "may," "will," "would," "could," "should," “might,”“poten-tial,” or "continue" and variations or similar expressions. Readers should not unduly rely on these forward-looking statements, which are not a guarantee of future performance. There can be no assurance that for-ward-looking statements will prove to be accurate, as all such forward-looking state-ments involve known and unknown risks, uncertainties and other factors which may cause actual results or future events to differ materially from the forward-looking state-ments. Such risks include, but may not be limited to: general economic and business conditions; technology changes; competi-tion; changes in strategy or development plans; governmental regulations and the ability or failure to comply with governmen-tal regulations; the timing of anticipated results; and other factors referenced in the Company’s business materials and prospec-tuses. |
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Management CEO Director Maurizio Chiriva-Internati, PhD Mr. Chiriva-Internati is an associate professor at MD Anderson Cancer Center. He has spent the past 28 years studying cancer targets and is the founder of Kiromic Artificial Intelligence Neural Network. He has published +160 articles (+peer reviews) on cancer targeting and on the use of AI to expedite the search for these targets. He holds PhD in immunology (U of Nottingham), PhD in morphological science (Milan), and a Certificate in Artificial Intelligence - M.I.T. CFO COO Director Tony Tontat Mr. Tontat brings to Kiromic over 2 decades of business experience from public (NASDAQ: SRNE, NK) and privately held biotechs. He had been healthcare analysts at specialist healthcare investment funds in New York, and Connecticut. He was also an investment banker at HSBC Securities in their New York, London, and Paris offices. Bachelor of Arts in Economics - Harvard University. CSIO Director Gianluca Rotino Chief Strategy, Innovation Officer Mr. Rotino held CEO and Chairman roles in several Italian companies specializing in high-tech, and corporate consulting. He also worked at law firms in Milan where he specialized in M&A, intellectual property prosecution and corporate law. He holds a business development degree and bachelor of science (electronics) - EBD Academy in London, and completed the drug discovery, develop. and commercialization - U.C. San Diego. CMO Scott Dalhbeck, MD, PharmD Dr. Dalhbeck was a radiation oncologist and was an adjunct professor of internal medicine, pathology, and urology at Texas Tech. He has also patented, manufactured, and commercialized IP and has more than a decade of experience in medical and oncology commerce. He holds an MD - Texas Health Science Center, and a PharmD - U of Nebraska. His residency was at Kaiser Permanente of Los Angeles. |
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Artificial Intelligence Neural Network for Target Selection dCAS9 OH gRNA Non-Viral Genome edit and delivery We are connecting the dots in cancer research by using AI and machine learning to connect silos of informations and arrive at cancer targets which will be more effective vs. classic development, saving man-years and billions in development dollars. Integrase ABBIE Our single-cut gene edits carry a lower mutagenesis risk vs. classic double-cut gene edits. Our CAR receptors will also have higher safety with an on-demand cut-off switch vs. classic CAR therapies with no off-switch. KiromicataGlance Revolutionizing Next-Gen Allogenic CAR Therapy for Solid Tumors Gamma Delta T-cellMicro Tumor Environment Immune Cell Type Our CAR Therapy will be using off-the-shelf Gamma-Delta T-cells and will have a higher yield and significantly lower yield variability vs. classic CAR-T therapies. Our CAR Therapies will be able to access the micro tumor environment due to our chPD-1 check-point activator vs. classic CAR-T therapies. Classic CAR-T are limited to hematologic indications. Solid Tumor |
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Artificial Intelligence Neural Network for Target Selection Diamond is a computational platform and a neural network that can identify new cancer immunological targets for T cells and B cells. Diamond is an artificial intelligence and machine learning approach that can identify novel surface tumor targets. It uses public and proprietary samples and can expand into the tumor target space. ADVANCING CAR through A.I. |
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Big Data Science BigDataScience meets TargetIdentification dramatically compressing Manual Target Identification Man-Years and Billions of Drug Development Dollars to develop a live drug |
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Artificial Intelligence Engine’s Compression of Time & Costs for live drug development Classic Chemistry Phase 1Phase 2Phase 3 Classic Small Molecules + Classic Targets Small Molecule A.I. Target Prediction CAR-Therapy Target Identification & Validation Pre-clinical Regulatory |
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Step 1 BigDatabases Public Databases The Cancer Genome Atlas Cancer immune peptides database Private A.I. Engine Servers |
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Step 2 ArtificialIntelligenceEngine +histo-aminochemistry filters, +machine learning Diamond ProprietaryDiamond Proprietary r-Epitope Epitope Selection iCloud Web Client Accessible Cancer Splice Iso-form Selection 3D Visualization ServersServers Iso-forms NASDAQ: KRBP Target Selection & Prediction 9 |
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Step 3 Prediction Heatmap of T-cell, B-cell epitopes Artificial Intelligence Selected Target A.I. Prediction High expression in cancer cells Low expression in normal cells Quantity surface antigen expression signature High affinity to TCR |
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Step 4 Target Validation We rigorously validate all targets from our A.I. Prediction Engine Internal validations and then external validation Wet Lab Validation Baylor University University of Rome Humanitas Research Hospital (Milan) Algorithm Validation Yale |
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Processes non-exhaustive list of functions being applied by A.I. Engine Prioritizing T and B Cell Targets Diamond generates a prioritized list of cancer immunological targets for T cells and B cells. These targets can be used to create therapies such as antibody therapies, T cell therapies, T cell receptor therapies, CAR T cell therapies and vaccine therapies. Identify Highly Expressed Genes Diamond’s cognitive and deep learning capabilities extract information from our extensive digital library consisting of clinical studies, genomic and proteomic datasets. Diamond harmonizes all the raw data and creates datasets which allows us to screen for cancer targets. Diamond will identify and prioritize lists of genes (biomarkers, wild type, mutant, isoform, neoepitope, etc.) that are highly and specifically expressed in the disease of interest while providing its distribution and methylation status across the entire patient population. It also maps out the exact portion of the gene that will elicit an immune response. Perform Meta Analysis Diamond performs meta-analysis and convolution studies while standardizing and normalizing data across multiple and variable experimental platforms, then allows for the visualization of consistent and accurate results in a user-friendly fashion. Predict Isoform Targets Cancer cells will down regulate or shed targets in order to avoid detection and destruction by T cells (the immune system). These variations are known as isoforms. CancerSplice also shows a box plot by tissue of expression of the isoform in normal cancer genome atlas tissues and a box plot of the matching isoform in genotype-tissue expression program normal data. The sequence of amino acids that are specific for the selected cancer isoforms are then directly fed to Diamond’s artificial neural capsule network for peptide design and prioritization. |
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CancerSpliceTM A key A.I. Engine Target isoforms are protein variants of the same targets that occur during the normal processing of immature gene transcripts to the mature form. Target isoforms include variations in their primary amino acid sequence that can change both the final folded form of the target plus their ability to be recognized by modified T cells (autologous/allogeneic) and other cells, such as NK or invariant NKT cells (often used in the allogeneic setting). If they are the predominate form on the cell surface, these isoforms can make it impossible for T cells to outright bind the targets on cancer cells. No binding or insufficient binding to the isoform results in no killing of cancer cells. Our CancerSplice accurately predicts the most appropriate isoforms for T cells to bind and destroy cancer cells. |
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Targets Which We Have Identified How our identified targets are developed into therapies for live drugs to treat cancer AIDT-1 Target Iso Mesothelin Iso Mesothelin TargetTarget Hematology MPM (Malignant Pleural Mesothelioma) Solid Tumor - Lung EOC (Epithelial Ovarian Cancer) Solid Tumor - Ovarian |
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Indications:By the Numbers Ovarian Cancer MPM - Lung Cancer Hematological Cancers 300,000 Worldwide number of patients 43,000 Worldwide number of patients 200,000 Worldwide number of patients American Cancer Society American Cancer Society American Cancer Society 21,750 2018 new cases in the USA 3,000 Annually in the USA 30,000 Annual Diagnosis in the USA American Cancer Society 2018 American Cancer Society 2018 American Cancer Society 2018 $1.2 BLN in 2018 $300 M by 2025 $4.6 BLN by 2025 Grand View Research (July 2019) Persistent Market Research, Jul 2017 BIS Research, Nov 2019 |
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OurPipeline In vitro validation Pre clinical IND Phase 1 Phase 2 Phase 3 Alexis (γδ-T cells) Allogenic / Iso-Mesothelin EOC (Solid, Ovarian) Alexis (γδ-T cells) Allogenic / Iso-Mesothelin MPM /Pleural mets (Solid, Pleural) Alexis (γδ-T cells) Allogenic / AIDT-1 (Hematologic Indications) chPD-1 check point activator (Solid Tumors) Check-Point Activation Isoform Mesothelin NASDAQ: KRBP Checkpoint inhibitors block PD-1 and PD-L1. Our chPD-1 (chimeric PD-1) takes it one step further by converting PD-1 and PD-L1 from an inhibitory signal to an activation signal. This pivotal CAR transformation allows our CAR T-cells to then kill solid tumors and TME (tumor micro environment). This is our lead target candidate which came out of our Artificial Intelligence Prediction Engine. Isoform targets are highly expressed on cancer cells while very lighly expressed on normal (healthy) cells. 16 |
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2020 Clinical Programs 202120222023 4Q4Q4Q Target chPD-1 Iso-Meso IND Proof of Concept Phase 1 PD-1 Iso-mesothelin chPD-1 + Iso-Meso Phase 1/2 Ovarian Dosing Efficacy, Observation Cell Type Gamma-Delta Cells sub-type of immune cells Solid Tumors: PD-1’s Role Classic CAR-T therapies are currently not being used in solid tumors. Solid tumors have PD-1s in their micro tumor environment. PD-1 put the “brakes” on T and NK Cells’ killing of antigens (tumor cancer cells). PD-1 + Iso-Meso Gamma-Delta Cells IND Phase 1 Safety Arm PD-1 + Meso chPD-1 + Iso-Meso Phase 2 MPM Lung Dosing Efficacy, Observation chPD-1 + AIDT1 Our chPD-1 (chimeric PD-1) takes it one step further by converting PD-1 and PD-L1 from an inhibitory signal to an activation signal. This pivotal CAR transformation allows our CAR T-cells to then kill solid tumors and TME (tumor micro environment). NASDAQ: KRBP Hematology Gamma-Delta Cells IND Phase 1 Safety Arm PD-1 + AIDT1 Phase 2 Hematology Dosing Efficacy, Observation 17 |
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ADVANCINGCARthroughA.I. Our Therapeutic Products AllogenicCAR Immuno CAR-GD-T Therapy in solid tumors |
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Allogenic Engineered Immune Therapy Step 01:Fractionation Healthy Donor Screening shows donor has healthy Gamma-Delta T cells Whole BloodFractionation Gamma-Delta T cells extracted |
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Allogenic Engineered Immune Therapy Step 02:Genome Edit dCAS9 GenomeEdit Gamma-Delta T cells OHgRNA Integrase ABBIE Proprietary Gene Edit Mechanism |
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ABBIE Gene Editing Technology Linear Non-viral Template dCAS9 LTR Promoter CAR Off-switch Anti-tumor LTR gRNA Integrase dCAS9 Figure 1. Our ABBIE gene-editing technology begins with the transgene template plasmid. Plasmid DNA is cut with restriction enzyme, ScaI, liberating the transgene template along with the retroviral-derived long-terminal repeats (LTRs), which is Figure 3. The guide RNA (gRNA) tethers ABBIE-bound template to the target site via DCas9, and Integrase helps to attach the exposed 3’OH groups to the target site on both strands without causing a dsDNA break. purified away from the plasmid DNA and ScaI protein. OH dCAS9 gRNA Integrase dCAS9OH Integrase ABBIE LTR LTR gRNA Figure 2. The ABBIE integrase, derived from HIV, is added, which binds to the LTRs and exposes a reactive 3’-OH group on each end. Figure 4. Following stable integration of the template into the target DNA locus, a short DNA duplication is present on each end. |
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Allogenic Engineered Immune Therapy Step 03:Gamma-Delta T Cells expanded invitro Chimeric Antigen Receptor CAR Receptor Expansion CAR CAR CAR CAR CAR Gamma-Delta T Cells start expressing CAR Receptors CAR Engineered Gamma-Delta T Cells expanded invitro |
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How We Know:GD-T cell Expansion Works γδ T cell phenotyping zoledronic acid +IL-2 Day 0 γδ T PBMC γδ T cell γδ T cell γδ T cell γδ T cell product Day 7 γδ T Before Enrichment PBMC Isolation initial expansion negative selection & transduction first expansion second expansion DAY 0DAY 7DAY 14 A large fold of expansion of highly pure γδ T cells during in vitro stimulation, culture, isolation and expansion process. Day 7 γδ T After Enrichment CD3+γ9δ2+T % 80% 60% γδ T Purity 15,000 10,000 γδ T Expansion SSC-H CD3 TCRVγ9 Day 14 γδ T Cells 40% 20% Folds of Expansion Pre-selection 14 Post-selection 5,000 Day 0714 CONCLUSION Our method of γδ T expansion yield highest 12,000-fold expansion of γδ T cells, which is over 95%purity for posi-tive for CD3, Vγ9, and Vδ2. The percentage of CD3+γ9+δ2+ T cells over 14-day culture. The expansion fold of CD3+γ9+δ 2+ T cells with our method. This has potential to produce enough number γδ T for clinical use. |
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Allogenic Engineered Immune Therapy Step 04:GD-T Cells infused into Patient CARCAR CAR CAR Off-The-Shelf Gamma-Delta T cells CAR CAR Engineered Gamma-Delta T cells Immune Cell Therapy Gamma-Delta T cells Patient receives engineered Immune Cell Therapy |
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Up-Armoring Accessory proteins can “up-armor” cellular therapies Strategic choice of proteins to improve cellular function and neutralize anti-immune responses Linear Non-viral Template *Activated signaling molecules chosen to enhance cell persistence by stimulating cytokine pathways LTR Promoter CAR Off-switch Anti-tumor LTR *Targeting the immunosuppressive “reactive” stroma can enable tumor targeting by therapeutic cells while increasing anti-tumor efficacy |
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dCAS9 OHgRNA Integrase Switches ABBIE ON OFF ON OFF ON OFF ACTIVATION SwitchATTENUATION Switch SAFETY Switch A rapidly deployed activation switch can provide a survival and proliferation signal to the therapeutic cells to enhance their efficacy and persistence in vivo. NASDAQ: KRBP A rapidly deployed attenuation switch can intercept activation signals transiently to minimize toxicity following successful anti-tumor interactions. Choice of two non-mutually exclusive Attenuation Switch approaches: (a)a protein-based switch that rapidly triggers attenuation of target cells in a dose-dependent fashion. (b) a small molecule-based approach to rapidly and reversibly attenuate cell signaling. A rapidly deployed, protein-based safety switch can eliminate therapeutic cells in case of acute toxicity.The safety switch is designed to eliminate either: (a)essentially all active therapeutic cells. (b)only the most active cells, preserving a cohort of backup therapeutic cells for long-term control of residual relapsing tumor cells. The Safety Switch will be co-expressed along with the bioactive chimeric activation receptor (CAR), the Activation Switch, and the Attenuation Switch.26 |
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dCAS9 OH gRNA Non-Viral Artificial Intelligence Neural Network for Target Selection We are connecting the dots in cancer research by using AI and machine learning to connect silos Integrase ABBIE Genome edit and delivery Our single-cut gene edits carry a lower mutagenesis risk vs. classic double-cut gene edits. Our CAR receptors will also have higher safety with an on-demand of informations and arrive at cancer targets which will be more effective vs. classic development, saving man-years and billions in development dollars. ValueDrivers Revolutionizing Next-Gen Allogenic CAR Therapy for Solid Tumors cut-off switch vs. classic CAR therapies with no off-switch. Our CAR Therapy will be using off-the-shelf Gamma-Delta T-cells and will have a higher yield and significantly lower yield variability vs. classic CAR-T therapies. Solid Tumor Our CAR Therapies will be able to access the micro tumor environment due to our chPD-1 check-point activator vs. classic CAR-T therapies. Classic CAR-T are limited to hematologic indications. Gamma Delta T-cellMicro Tumor Immune Cell Type Environment NASDAQ: KRBP27 |
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Comparables A.I. Targets + Small MoleculesCAR-T and CAR-NK $4.18 BLN IPO Feb 2020 $1.14 BLN IPO Jan 2020 $4.20 BLN Public $3.10 BLN Public $1.04 BLN IPO Mar 2018 $110.7 M $11.9 BLN Acquired $9.0 BLN IPO Jun 2020 Acquired NASDAQ: KRBP Market data as of 10/23/2020, Yahoo Finance closing28 |
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THANK YOU 7707 Fannin Street, Suite 140 Houston, Texas 77054 +1 (806) 368 - 6731 ttontat@kiromic.com |
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% Survival dCAS9 OH gRNA Integrase Effector Cell PD1 Extracellular Domain PDL-1 ABBIE Pending Artificial Intelligence Neural Network for Target Selection Granted 9149441: Nanospheres Encapsulating Bioactive Material and Method for Formulation of Dap10 Costimulatory Domain CD3ζ Signaling Domain CD28 Transmembrane Domain Pending Cancer Cell PCT/US2016/025426: CAS 9 Retroviral Integrase and CAS 9 Recombinase Systems for Targeted Incorporation of a DNA Sequence into a Genome of a Cell or Organism Nanospheres Pending PCT/US2018/052799: PD1-Specific Chimeric Antigen Receptor as an Immunotherapy 15/731,143: Platform for Identification of Tumor-Associated Cancer/Testis Antigens Switch Technology Pending Provisional Patent Application No.: 63/039,364 - Tri Switch Technology for Multi-Dimensional Control of Cell Therapy PCT/US20/35183: Methods for Identifying and Using Diseases-Associated Antigens 15/530,964: Anti-Human/Mouse Sperm Protein 17 (SP17) Antibody and Derivatives Thereof PCT/US2017/022168: Compositions and Methods for Treating Cancers 15/932,396: CdS Quantum Dot-Chitosan-Anti-SP17 Nanohybrid as a Potential Cancer Biomarker PCT/US2015/061703: Novel Nanoparticle—Based Vaccine Targeting Cancer/Testis Antigens (CTA) and its' Use in Solid and Hematological Malignancies 10004790: Nanospheres Encapsulating Bioactive Material and Method for Formulation of Nanospheres Gamma-Delta T-cells Pending 63/048,488. Mesothelin Isoform Binding Molecules and Uses Thereof |
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Directors Tony Tontat Maurizio Chiriva-Internati, PhD Gianluca Rotino Independent (Board Nominee)Independent (Board Nominee)IndependentIndependent Jerry Schneider, JD, MBAMichael NagelAmerico Cicchetti, PhDPietro Bersani, CPA Mr. Schneider has been nominated to serve on our Board of Directors. He currently serves on the board of directors and audit committee for Cognex, a provider of vision systems, software, sensors, and industrial barcode readers used in manufacturing automa-tion since 2016. Cognex (CGNX) is publicly traded on the Nasdaq stock exchange. He serves on other for-profit and non-profit boards. Mr. Schneider received his Juris Doctor from Loyola Law School, and a B.S. in Accounting from the University of California at Berkeley. He has experience of being a ‘‘financial expert’’ appointed by the U.C. Regents which oversee the University of California’s budget of over $30M. Mr. Nagel has been nominated to serve on our Board of Directors. Mr. Nagel has over 30 years of sales and marketing experience in the medical device industry. Since 2012, Mr. Nagel has served as the President and CEO of Vomaris Innovations, Inc, which specializes in wireless microcurrent-generating technologies that are focused on regeneration, healing, and recovery. Previously, Mr. Nagel served as the Chief Commercial Officer of Neomend, a biomaterial company that developed ProGel, a PMA approved surgical sealant for lung surgery. From 1997 to 2005, Mr. Nagel also served as Co-Founder and Vice President of Worldwide Sales and Marketing at Vascular Solutions (VASC). In addition to Mr. Nagel’s executive experience, he also serves as a director for Franklin Mountain Medical, LLC an early stage company in the structural heart market. Mr. Nagel holds both a B.A. in Business and a M.B.A. from the University of St. Thomas. Dr. Cicchetti has served as a member of our board of directors since March 2020. Dr. Cicchetti has served as a Professor of Management at Universit`a Cattolica del Sacro Cuore, Faculty of Economics, Rome since 2006. He is also currently the Director of the Graduate School of Health Economics and Management at Universit`a Cattolica del Sacro Cuore. In addition to his academic experience, Dr. Cicchetti was a member of the Price and Reimbursement Committee of the Italian National Drug Agency from 2009-2015. He is a member of the European Network of Health Technology Assessment; Member of the Innovation Steering Group of the National HTA Program for Medical Devices (Ministry of Health, Italy); Member of the National Immunization Technical Advisory Group at the Ministry of Health, Italy since 2019; Member of the Health and Research Commission of the Rome Foundation since 2007; and a Member of the Board of Directors of the Health and Research Foundation since 2017. Furthermore, Dr. Cicchetti is the Chief Executive Officer and Director for Molipharma, whose core business is the research and development of new drugs and diagnostics aimed at predicting, detecting and treating female oncological diseases. He also serves as an independent board member for Foundation Health and Research, and Leonida SICAF, a fixed capital investment company. He obtained his PhD in Management from University of Bologna, and his B.A. from University of Rome. Mr. Bersani has served as a member of our board of directors since June 2020. Since April 2020, Mr. Bersani is a Partner with B2B CFO Partners, LLC, which provides strategic management advisory services to owners of privately held companies. During October 2016 and July 2018, he served as the President, and Chief Executive Officer at K.P. Diamond Eagle, Inc., a consulting firm specialized in development of innovative commercial and private aviation business models. He also held the same positions at K.P. Diamond Eagle, Inc. between November 2019 and March 2020. He later served as a Senior Director within Alvarez & Marsal’s Private Equity Performance Improvement Practice, LLP between August 2018 and October 2019. Prior to those professional experiences, Mr. Bersani served as the Chief Financial Officer of Fuel Systems Solutions, Inc. between April 2011 and October 2016. Mr. Bersani is a Certified Public Accountant and is also a Certified Public Auditor and a Chartered Certified Accountant in Italy where he developed a significant knowledge of US GAAP and IFRS. Mr. Bersani earned a BA and MA in Business Economics from L. Bocconi University, Italy. Mr. Bersani was designated by certain holders of our Series B Preferred Stock. Except for the foregoing, there is no arrangement or understanding between any director or executive officer and any other person pursuant to which he was or is to be selected as a director. |
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Artificial Intelligence Neural Network 162 Internal Publications Chiriva-Internati M, Cobos E, Cannon MJ. Editorial: Prospects and Chal-lenges for Immunotherapy of Ovarian Cancer-What Can We Learn from the Tumor Microenvironment? International Reviews of Immunology. 2011;30(2-3):67-70. Maurizio Chiriva-Internati, Leonardo Mirandola, Franco Marincola, Gianluca Rotino, Jose A. Figueroa, Fabio Grizzi, and Robert Bresalier The Quest for the Next-Generation of Tumor Targets: Discovery and Prioritization in the Genomics Era. Springer, Published 2019.( chapter for a book “immune-Oncology: Cellular and Translational Approaches” 2019 Cannon MJ, Goyne H, Stone PJB, Chiriva-Internati M. Dendritic cell vaccination against ovarian cancer - tipping the Treg/T(H)17 balance to therapeutic advantage? Expert Opinion on Biological Therapy. 2011;11(4):441-445. Maurizio Chiriva-Internati & Adrian Bot. New Era in Cancer Immunotherapy: Discovering Novel Targets and Reprogramming the Immune System. International Reviews of Immunology, 34:2, 101-103. 2015 Wachtel MS, Zhang Y, Xu T, Chiriva-Internati M, Frezza EE. Combined hepatocellular cholangiocarcinomas; analysis of a large database. Clin Med Pathol.1:43-7. 2008 Grizzi F, Gaetani P, Tancioni F, Di Ieva A, Bollati A, Baena R, Dioguardi N, and Chiriva-Internati M. From Discovery to the Clinical Application In Nervo System Neoplasia. In: Tumor Associated Antigens, 2004. Mirandola L, J Cannon M, Cobos E, Bernardini G, Jenkins MR, Kast WM, Chiriva-Internati M. Cancer testis antigens: novel biomarkers and targeta-ble proteins for ovarian cancer. Int Rev Immunol. Apr-Jun;30(2-3):127-37. doi: 10.3109/08830185.2011.572504. 2011 Bumm K, Zheng M, Bailey C, Zhan F, Chiriva-Internati M, Eddlemon P, Terry J, Barlogie B, Shaughnessy JD Jr. CGO: utilizing and integrating gene expression microarray data in clinical research and data management. Bioinformatics. 2002 Feb;18(2):327-8.2002 Figueroa AJ ,Pena C ,Mirandola L, Reidy A , Payne D, Hosiriluck N, Suvorava N, Rahman LR , Whitlow AR ,Verma R , Cobos E and Chiriva-Internati M. “Therapeutic Monoclonal Antibodies and Their Targets” by Wiley Production_Biosimilars of Monoclonal Antibodies: A Practical Guide to Manufacturing, Preclinical and Clinical Development, First Edition. Edited by Cheng Liu and K. John Morrow Jr. © 2017 John Wiley & Sons, Inc. Published 2017. Grizzi F, Russo C, Portinaro N, Hermonat PL, Chiriva-Internati M. Complexity and cancer. Gastro-enterology. 2004 Feb;126(2):630-1; author reply 631-2. PubMed PMID: 14765401.2004 NASDAQ: KRBP Source: Pubmed.gov, search Chiriva-Internati (162 publications from 1997 to 2020) 32 |
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ABBIE: Non-Viral Genome Editing Mechanism ABBIE is a novel gene-editing system for inserting therapeutic genes safely into the genome of a host cell. ABBIE technology comprises two main components, (i) a genome template (extracted from the ALEXIS plasmid), containing the therapeutic genes needed to retrain tumor-killing cells, and (ii) the gene-editing machinery required to safely insert this template into the genome of the therapeutic cells. The ABBIE protein accompanies the CAR-containing genome template as it passes through the cell membrane into the nucleus and guides the template-flanking sequences (the “glue”) safely into the target genome. Due to this targeting ability, ABBIE can also be used to remove unwanted, inhibitory genes. CAR expression on the Gamma-Delta T cells allows them to detect and destroy the antigen-expressing targeted cells. The OFF switch permits fast shutdown in the event of an unexpected toxicity. Additional Anti-tumor factors can help neutralize the toxic tumor microenvironment. NASDAQ: KRBP33 |